Abberior STEDYCON is a revolutionary microscopy system that transforms your standard microscope into a high-resolution imaging tool. Thanks to STED (Stimulated Emission Depletion) technology, STEDYCON allows you to go beyond traditional diffraction limits, providing nanometer-level resolution. It is the perfect solution for researchers seeking to push the boundaries of fluorescence microscopy. However, if for some reasons the STEDYCON super resolution feature is not enough, you can now push it even further.
In fact, with FLIM LABS Upgrade you can now add another level of information to your super-resolution images.
Fluorescence Lifetime Imaging Microscopy (FLIM) is an advanced imaging technique that measures the decay time of a fluorophore’s excited state, also called Fluorescence Lifetime. This lifetime is sensitive to the local environment, such as pH, ion concentration, or protein interactions, making FLIM highly useful for studying cellular processes, metabolic state, protein-protein interactions, bindings, etc. Unlike intensity-based fluorescence imaging, FLIM is less affected by variations in fluorophore concentration, light scattering, or photobleaching, but most importantly exploits auto-fluorescence signals. Altogether, these features make FLIM a valid approach for many applications, providing a new layer of information on intensity-based imaging.
Even though super-resolution FLIM is clearly a cutting-edge technology, achieving it, it is extremely simple. Thanks to FLIM LABS devices, upgrading Abberior STEDYCON is straightforward. FLIM LABS is an Italian company specialized in manufacturing Fluorescence Lifetime devices and software. The modular design of their devices enables instant compatibility with the microscope, bringing time-resolved fluorescence imaging into your lab with minimal setup. Moreover, FLIM LABS provides an acquisition software that seamlessly interfaces with the Abberior hardware, automatically recognizing key signals such as Pixel, Line, and Frame Clocks for precise image reconstruction (Figure 1)
Figure 1: Graphical representation of FLIM LABS hardware and software setup. The compact design and small dimensions of FLIM LABS devices make the installation straightforward, while the user-friendly software allows instant super-resolution FLIM acquisition and analysis.
Whether you are a beginner or an advanced user, FLIM LABS hardware and software have you covered with their easy setup. The integration process is simple and fast, making advanced super-resolution FLIM accessible to
